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The Mitochondria Panel (DHS-105Z) workflow launched using the Analyze QIAseq Panel tool, uses the standard genetic code rather than the vertebrate mitochondrial code in affected software versions. Used as delivered in affected versions, this affects predictions based on codons that differ between these 2 codes. Of particular note is the potential for false positives or false negatives relating to whether a variant causes premature translation termination, due to incorrect nonsense annotations.
In addition, the Genetic Code parameter of the Amino Acid Changes tool in the Ready-to-Use workflows is locked to “Standard” in affected software versions. Thus, when using such workflows, the suggested work-around should also be used when analyzing data from the QIAseq Mitochondria Panel DHS-105Z.
– Biomedical Genomics Analysis 1.0
– QIAseq Targeted Panel Analysis 1.0, 1.0.1, 1.1 and 1.2
– QIAseq DNA V3 Panel Analysis 1.0 and 1.0.1
This issue was fixed in Biomedical Genomics Analysis 1.1 and the corresponding server plugin.
The work-around is documented at:
This work around can be carried out in any of the affected software versions.