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The value for the “Mean read coverage per reference gene control primer” in QC for RNAscan Panels reports generated using the Biomedical Genomics Analysis 20.2 plugin is 100 times too high. This has the knock-on effect that the “Target gene vs reference gene coverage (%)” value in these reports is 100 times too low.
For reports generated using Biomedical Genomics Analysis 20.2,
These adjustments should not be applied to reports generated by earlier versions of the Biomedical Genomics Analysis plugin, as those are not affected by this issue.
Biomedical Genomics Analysis 20.2. The following ready-to-use workflows include QC for RNAscan Panels:
Earlier versions of this plugin are not affected, and the issue has been addressed in Biomedical Genomics Analysis 21.0.