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Long Read Support

The Long Read Support (beta) plugin provides a range of tools for working with long, error-prone reads, typically produced by PacBio or Oxford Nanopore sequencing technologies. Specifically, this plugin provides functionality for:

  • Correcting errors in long reads
  • Assembling long reads
  • Mapping long reads against a reference
  • Polishing contigs

 

Screenshot of the tools added to the toolbox after installation of the plugin.

 

The tools delivered by this plugin use the  minimap2, miniasm and racon open source software components, and can be used with equal ease on supported Windows, macOS and Linux systems.

Results can be further processed by other tools of the QIAGEN CLC Genomics Workbench, QIAGEN CLC Genomics Server and relevant plugins. For example, the initial contigs obtained by assembling error-prone, long reads may be further refined using tools delivered by the QIAGEN CLC Genome Finishing Module.

 

Known limitations and system requirements

  • These tools are optimized for working with small and medium-sized genomes (up to 20M base pairs). They are not designed for larger eukaryotic genomes.
  • Systems require CPU with an AVX instruction set to use these tools.

Long Read Support is a plugin for QIAGEN CLC Genomics Workbench. The functionality is also available for QIAGEN CLC Genomics Server using the Long Read Support Server Plugin. Learn more about de novo assembly using long reads and short read polishing in this tutorial.

We welcome your feedback and suggestions. Please get in touch by emailing ts-bioinformatics@qiagen.com.

 

Downloads

Plugin Manual
Online manual Download manual
Plugin Download
Download plugin
Download Long Read Support (Beta)

Version

Platform support

Download

20.0.0 Beta v.1

QIAGEN CLC Genomics Workbench


 [20.0.4, 20.0.3, 20.0.2, 20.0.1, 20.0]
Server Plugin Download
Download plugin
Download Long Read Support Server Plugin (Beta)

Version

Platform support

Download

20.0.0 Beta v.1

QIAGEN CLC Genomics Server


 [20.0.4, 20.0.3, 20.0.2, 20.0.1, 20.0]

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