CLC LightSpeed Module 23.0.2

Released on 25.05.2023

Bug fixes

• Fixed an issue that caused LightSpeed Fastq to Germline Variants to assume that fastq files for paired reads were provided in the order R1, R2, and that read pairs were provided consecutively.
  If R1 and R2 were swapped for a given read pair, the same variants would be called, but the direction of the paired reads in the mapping was reverse of what it should have been.
  If R1 and R2 were swapped between different read pairs leading to a different number of reads in R1 and R2, the tool would hang indefinitely producing no results.
  Windows users are likely not affected, because the system alphabetically re-orders fastq files. Mac users can be affected because the order that files are selected is retained when starting an analysis.
• Various minor bug fixes

CLC LightSpeed Module 23.0.1

Released on 23.02.2023

Improvements

• Speed improvement to outputting read tracks from LightSpeed Fastq to Germline Variants.

Bug fixes

• Fixed issues affecting LightSpeed Fastq to Germline Variants that could cause variant count, coverage and frequency to be incorrect:
  • When the option “Ignore non-specific matches” was used, non-specific matches were still included when calculating count, coverage and frequency. Variants in regions with ambiguously mapped reads were affected.
  • Broken read pairs were included when calculating count, coverage and frequency. Variants in regions with broken reads were affected.
  • Variant calls and zygosity were not affected by the issues, see Important QIAGEN CLC software notifications for further details.
• Various minor bug fixes

CLC LightSpeed Module 23.0

Released on 17.01.2023

This is the first release of this product.